Translational_Unit
Part:BBa_K4773010:Design
Designed by: Yan Pan Group: iGEM23_UESTC-China (2023-10-01)
RBS-ATF1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 544
Illegal SapI.rc site found at 1552
Design Notes
The RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts. There are SacI and SalI restriction sites at the end of the ATF1 gene, which can be used for downstream manipulation of the part.
Source
This part is derived from the genomic sequence of Saccharomyces cerevisiae, a gram-positive eukaryotic yeast that is widely used in baking and brewing.